Mycoplasma bovis testing and surveillance

To build a picture of where Mycoplasma bovis is, we're tracing animal movements and testing samples from dairy farms across New Zealand. Read about the testing process.

Tracing the disease

A large operation is underway to build a picture of where Mycoplasma bovis is and help manage it.

Testing for Mycoplasma bovis

Mycoplasma bovis is difficult to find. Infected cattle appear completely normal most of the time and they shed the bacteria intermittently. So when we test for Mycoplasma bovis, we try to improve the chances that we will find an infected herd by:

  • testing large numbers of animals
  • testing multiple times.

It means testing a lot of animals from all the different "in-contact" groups on your farm and testing those groups multiple times. This is why testing programmes for each farm take some time and involve several tests.

Testing farms connected to Mycoplasma bovis

We are testing all of the farms that are connected in some way to the farms we currently know have tested positive for Mycoplasma bovis. This includes neighbouring farms and those who have received animals from farms which are under investigation or infected properties.

A farm is a forward trace if the Ministry for Primary Industries (MPI) finds they have received animals, milk, or other risk goods from a farm of interest, which is a Restricted Property (RP) or an Infected Property (IP).

We find most of our traces by looking at:

  • NAIT (National Animal Identification and Tracing) movements from the farm of interest
  • RP/IP farmer written reports
  • public reports from farmers who have a movement that is not recorded in NAIT.

These movements are entered into our database. Our casing team will then call the trace farm to check the details and confirm whether they received the risk movement. Calls from farmers are also entered into the database at this point. This is when farms hear that they are a trace farm.

From trace to surveillance

The surveillance team will then review the information:

  • where the risk movement is from
  • when it happened
  • whether the trace animals are still on the farm
  • whether any animals on the farm were in contact with the trace.

Depending on this information and risk analysis, some farms are ruled out and removed from the trace list. If more investigation is needed, the farm will hear from us to book in testing.

When the call is made to book the first round of testing, a trace farm will know that they are under surveillance.

Unfortunately, MPI doesn’t have enough people to call every trace farm to tell them that they are not under surveillance, but if you are under surveillance you will get that second call to book testing. The timeframe depends on the urgency – higher risk farms will be contacted more quickly. We aim to call to book testing within 1 or 2 days from knowing a farm needs to go under surveillance.

How surveillance visits work

First visit

  • We visit the farm to take blood and swab samples from a selection of animals. To get the best possible results, we take blood tests from at least 100 animals, or for smaller herds, as many as needed to provide a good, clear result. We may also take bulk milk samples for in-milk dairy farms. 
  • The samples are sent to MPI’s Animal Health Laboratory in Wellington for testing.
  • The tests take up to two weeks to complete and farmers are told results as soon as possible.
  • If these tests are negative it does not mean a farm is definitely free of Mycoplasma bovis – a further one or two rounds of testing will be required to confirm that status.
  • If the tests indicate a positive result, this means more testing to confirm one way or the other.
  • This process can be frustrating for farmers, but we need to have total confidence in the results.

Second visit

  • After approximately 4 weeks, the tester will return to the farm and again test 100 animals. This will be a second random group of animals, which may or may not include some animals from the first round.
  • We need to do a second round even if the first test result was clear, because we haven’t tested all the animals and some animals can be carrying the bacteria but not be showing symptoms. If the disease is present, by the time of this second testing we would expect a greater number of animals to be infected and for this to show up in our tests.
  • Once again, it will take approximately 4 weeks for results to come through.

Third and ongoing visits

  • In the majority of cases, 2 rounds of testing are sufficient to determine the status of the farm. But if there is any uncertainty or not enough information, further rounds of testing may be needed.

The results

We aim to have results from any round of testing available to report to farmers within approximately 4 weeks of samples being taken.

When the lab has processed the samples the results will be put into our database. Only when we have all the results available in the database will the Surveillance team review them. After they have been reviewed, we will call to report out the results, and the next visit can be scheduled.

If you haven’t heard about your results after 5 weeks, you can contact MPI to ask after them. If you have an urgent situation and need your results, we will endeavour to put a priority on your results.

What happens if a farm has infected animals?

  • MPI will place a legal Restricted Place Notice on the property restricting the movement of animals and other risk goods off the farm.
  • You will be given an ICP Manager for all your MPI questions and support will also be available through your Rural Support Trust.
  • MPI will work with local vets to advise the farmer on managing any sick animals.

The methods for testing for Mycoplasma bovis

There are various types of tests used worldwide for Mycoplasma bovis. Due to the nature of Mycoplasma bovis as a disease and the difficulties in interpreting results, only the PCR is available for commercial use in New Zealand at this time.

The PCR test

This is the best test for individual animals. The PCR (polymerase chain reaction) test works by finding DNA from a virus or bacteria. This test is best able to detect Mycoplasma bovis when the animal is shedding.

Infected cattle will definitely shed when showing clinical signs of illness, but may also sometimes shed without showing signs of illness. If an animal is not shedding the bacteria, its DNA will not be present in the sample, and the test will be negative.

It can be used on milk samples, swabs from the back of the throat (hard to get), fluid from swollen joints of clinical animals, and semen or preputial swabs in bulls. It can’t be used on blood samples.

The PCR is excellent at letting us know when bacteria are present, but only when the animal is shedding. So you also have to take other factors into account when choosing to purchase animals. Do not base your decision only on the result of a laboratory test.


The Mycoplasma bovis ELISA test is designed to find antibodies to the bacteria in the animal’s blood. This means that, unlike the PCR, the ELISA is looking for the body’s immune response to an infecting organism, rather than looking for the organism itself. The ELISA can give information at the herd level by looking at how a group of animals’ immune systems are reacting to a bug like Mycoplasma bovis, which helps us determine if it is present in the herd. The ELISA test for Mycoplasma bovis is not as useful in the individual animal or in small groups of animals.

This test is not available for commercial use in New Zealand at this time but MPI is using it as part of our testing regime.

Testing your animals yourself

Testing your animals yourself may not be the best use of your time. Other things you can do to make a difference include fewer cattle movements and lower risk practices around feeding calves.

For any tests to be meaningful, they must be carried out on an adequate number of animals, according to the provided recommendations, at an appropriate time, and they should be interpreted as part of a larger picture including all available information about herd composition, movements, and health history.

Test results for Mycoplasma bovis can be used as part of your decision-making process for purchasing or leasing animals, but make sure you consider other factors that could influence risk of exposure.

Your veterinarian can help you understand testing for your group of cattle and can help you interpret the results.

Information sheet

The information on this page is also available in a document.

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