∙ N2O chamber measurements are often used for estimating N2O emission factors (EFs).
∙ Accurately determining EFs is challenging due to the inherently variable nature of N2O fluxes.
∙ We provide guidance on statistical analysis and EF calculation of N2O chamber measurements.
∙ Accurate reporting of data ensures the robustness of N2O results can be reliably evaluated.
∙ Authors should adhere to minimum requirements for reporting of experimental and metadata.